The typical peptide synthesis workflow is a multi-step process which involves synthesis, purification and evaporation with final freeze drying of the peptide product. This demands that the steps from amino acid building blocks to final purified peptide product proceed as efficiently and smoothly as possible. Solid phase peptide synthesis is becoming more routine with the advent of better synthesis instrumentation utilizing elevated temperatures, now even more complex and difficult sequences can be made. Although the synthesis step is the most important part of the workflow, purification and evaporation issues can dramatically impact the efficiency of the workflow and are the cause of many bottlenecks.
Preparative reversed-phase HPLC has normally been the method of choice for purification of synthetic peptides, but is limited by small loading amounts, long separation times, poor recoveries and high costs. Is there an alternative?
I'll present strategies to improve the peptide synthesis workflow, which enable the synthesis of highly pure crude peptides, demonstrate alternative techniques to rapidly purify crude peptide mixtures and highlight methods to rapidly concentrate solutions along the entire peptide synthesis workflow.