Oral Presentation 6th Modern Solid Phase Peptide Synthesis & Its Applications Symposium 2017

Graphical and SERS encoding system for on-bead screening of peptoid libraries (#4)

Yoon-Sik Lee 1 , Cheolhwan Jeong 1 , Dae-Hong Jeong 1
  1. Seoul National University, Seoul, South Korea

Screening of compound libraries remains one of the most challenging tasks in drug discovery, biomarker detection, and biomolecular profiling processes. Among various encoding methods for the identification of specific compound in library, advantage of surface-enhanced Raman scattering (SERS) based encoding lies in the availability of a large number of Raman active molecules which can be utilized as Raman label compounds. Moreover, the SERS signals have narrow band width (< 2 nm) without peak overlap, and a single excitation is used for the detection of multiple encodings. For peptoid library encoding, we have developed a simple and efficient SERS based encoding method on TentaGel beads, which involves disk type graphical and SERS encoders (so-called micro-encoders). The size of micro-encoders is 5 µm, and 1 µm-sized holes are located in the center of micro-encoders to produce graphical patterns. Also, characteristic SERS signals can be generated from the assembled Ag NPs on the micro-encoders. Based on these graphical and SERS two-dimensional approach, the sequence and the type of peptoid side chain could be encoded by the absorption of micro-encoders on the TentaGel beads. Using the graphical pattern and SERS signals from micro-encoders, we could identify the on-bead bioactive peptoid ligands which have binding affinity for a specific protein. We believe that our graphical and SERS encoding technology is a promising tool in the screening of one-bead-one-compound (OBOC) libraries for drug discovery.